let every_vc_on_fastqs ~reference_build ~normal ~tumor =
let aligner which_one =
Bfx.lambda (fun fq -> which_one ~reference_build fq) in
let align_list how (list_of_fastqs : [ `Fastq ] list Bfx.repr) =
Bfx.list_map list_of_fastqs ~f:how |> Bfx.merge_bams
in
let aligners = Bfx.list [
aligner @@ Bfx.bwa_aln ?configuration: None;
aligner @@ Bfx.bwa_mem ?configuration: None;
aligner @@ Bfx.hisat ~configuration:Biokepi.Tools.Hisat.Configuration.default_v1;
aligner @@ Bfx.hisat ~configuration:Biokepi.Tools.Hisat.Configuration.default_v2;
aligner @@ Bfx.star ~configuration:Biokepi.Tools.Star.Configuration.Align.default;
aligner @@ Bfx.mosaik;
aligner @@ (fun ~reference_build fastq ->
Bfx.bwa_aln ~reference_build fastq
|> Bfx.bam_to_fastq `PE
|> Bfx.bwa_mem ?configuration: None ~reference_build
);
] in
let somatic_of_pair how =
Bfx.lambda (fun pair ->
let normal = Bfx.pair_first pair in
let tumor = Bfx.pair_second pair in
how ~normal ~tumor ())
in
let somatic_vcs =
List.map ~f:somatic_of_pair [
Bfx.mutect
~configuration:Biokepi.Tools.Mutect.Configuration.default;
Bfx.mutect2
~configuration:Biokepi.Tools.Gatk.Configuration.Mutect2.default;
Bfx.somaticsniper
~configuration:Biokepi.Tools.Somaticsniper.Configuration.default;
Bfx.strelka
~configuration:Biokepi.Tools.Strelka.Configuration.default;
Bfx.varscan_somatic ?adjust_mapq:None;
Bfx.muse
~configuration:Biokepi.Tools.Muse.Configuration.wes;
Bfx.virmid
~configuration:Biokepi.Tools.Virmid.Configuration.default;
]
in
let aligned_pairs
: (([ `Fastq ] list * [ `Fastq ] list) -> ([ `Bam ] * [ `Bam ]) list) Bfx.repr =
Bfx.lambda (fun pair ->
Bfx.list_map aligners ~f:(
Bfx.lambda (fun (al : ([ `Fastq ] -> [ `Bam ]) Bfx.repr) ->
Bfx.pair
(align_list al (Bfx.pair_first pair : [ `Fastq ] list Bfx.repr))
(align_list al (Bfx.pair_second pair))
)
)
)
in
let vcfs =
Bfx.lambda (fun pair ->
List.map somatic_vcs ~f:(fun vc ->
Bfx.list_map (Bfx.apply aligned_pairs pair) ~f:(
Bfx.lambda (fun bam_pair ->
let (||>) x f = Bfx.apply f x in
let indelreal =
Bfx.lambda (fun pair ->
Bfx.gatk_indel_realigner_joint
~configuration: Biokepi.Tools.Gatk.Configuration.default_indel_realigner
pair)
in
let map_pair f =
Bfx.lambda (fun pair ->
let b1 = Bfx.pair_first pair in
let b2 = Bfx.pair_second pair in
Bfx.pair (f b1) (f b2)
)
in
let bqsr_pair =
Bfx.gatk_bqsr
~configuration: Biokepi.Tools.Gatk.Configuration.default_bqsr
|> map_pair in
let markdups_pair =
Bfx.picard_mark_duplicates
~configuration:Biokepi.Tools.Picard.Mark_duplicates_settings.default
|> map_pair in
bam_pair ||> markdups_pair ||> indelreal ||> bqsr_pair ||> vc
||> Bfx.lambda Bfx.to_unit
)
)
)
|> Bfx.list
)
in
let workflow_of_pair =
Bfx.lambda (fun pair ->
Bfx.list [
(Bfx.apply aligned_pairs pair
|> Bfx.list_map ~f:(Bfx.lambda (fun p ->
Bfx.pair_first p
|> Bfx.stringtie
~configuration:Biokepi.Tools.Stringtie.Configuration.default)
)) |> Bfx.to_unit;
Bfx.apply vcfs pair |> Bfx.to_unit;
begin
Bfx.apply
(Bfx.lambda (fun p -> Bfx.pair_first p |> Bfx.concat |> Bfx.seq2hla))
pair
|> Bfx.to_unit
end;
begin
Bfx.apply
(Bfx.lambda (fun p -> Bfx.pair_first p |> Bfx.concat |> Bfx.fastqc))
pair
|> Bfx.to_unit
end;
begin
Bfx.apply
(Bfx.lambda (fun p -> Bfx.pair_second p |> Bfx.concat |> Bfx.optitype `RNA))
pair
|> Bfx.to_unit
end;
begin
Bfx.apply aligned_pairs pair
|> Bfx.list_map ~f:(Bfx.lambda (fun p ->
Bfx.pair_first p
|> Bfx.gatk_haplotype_caller
))
|> Bfx.to_unit
end;
]
|> Bfx.to_unit
)
in
Bfx.apply workflow_of_pair (Bfx.pair normal tumor)